Oligo (dT)18 Primer is single-stranded sequence of deoxythymine (dT), used for priming reactions catalysed by reverse transcriptase. The transcript is primed in the poly(A) tail of mRNA molecules.
Oligo (dT)18 Primer is suitable for use as a primer for first strand cDNA synthesis with a reverse transcriptase. The primer hybridizes to the poly-adenylated tail found on the 3´ end of most eukaryotic mRNAs. Oligo (dT)18 ensures that the 3´ end of mRNAs are represented.
Primer sequence: 5´-d (TTT TTT TTT TTT TTT TTT)-3´
A mixture of random hexamer primers and oligo(dT) may improve the sensitivity of cDNA synthesis.
Bioline美国BIOLINE 美国BIOLINE公司:分子生物学试剂,生产以重组的脂质体转染试剂及PCR和RT-PCR产品闻名于世。成立于1992年,总部位于分子生物学领域的出色质量和可靠性试剂,包括dNTP,聚合酶和定量梯