Highly quality dNTPs are& a vital requirement for successful PCR, as the presence of contaminating impurities will result in a decrease in amplification sensitivity and product yield. Bioline ultra-pure dNTPs undergo highly stringent purification steps to give a greater than 99% purity and are tested for the absence of DNase, RNase, Protease, Nickase activity. They are then quality controlled in a variety of applications, ensuring that they are highly suitable for the most sensitive of PCR techniques.
dUTP in the mix is used in place of dTTP and is used in PCR and qPCR protocols to prevent carryover from previous amplifications. Uracil DNA Glycosylase (UDG) is added to a PCR premix to catalyze the release of uracil from uracil-containing single-stranded or double-stranded DNA created in previous PCR reactions, thereby preventing false positives.
Bioline dUTP Mix are supplied as 10 mM of ultra-pure dATP, dCTP and dGTP and 20 mM dUTP solution as lithium salts. Lithium salts have greater resistance to repeated freezing and thawing cycles than sodium salts and remain sterile due to the bacteriostatic activity of lithium towards various microorganisms, giving greater reliability and an extended shelf life.
As an ISO 13485 certified primary manufacturer of nucleotides, if your requirements for nucleotides are beyond the scope of our standard product range, Bioline can also offer custom, bulk and OEM services (custom@bioline.com).
Bioline美国BIOLINE 美国BIOLINE公司:分子生物学试剂,生产以重组的脂质体转染试剂及PCR和RT-PCR产品闻名于世。成立于1992年,总部位于分子生物学领域的出色质量和可靠性试剂,包括dNTP,聚合酶和定量梯