Description
Uracil DNA Glycosylase (UDG) catalyzes the release of uracil from uracil-containing single-stranded or double-stranded DNA, but not from RNA or oligonucleotides (6 or fewer bases).
UDG is active over a broad pH range with an optimum at pH 8.0, does not require a divalent cation, and is inhibited by high ionic strength (>200 mM).
Meridian UDG is purified to SDS-PAGE purity and is free of endonucleases, exonucleases, nickases and RNases.
Source: Recombinant E. coli strain carrying the over expressed gene of Uracil DNA Glycosylase from E. coli.
Product Highlights
- Removes uracils from DNA - causes fragmentation of DNA and stops amplification in a PCR
- Easy-to-use - novel, sensitive mutant is irreversibly inactive after heating
Product Description
Uracil DNA Glycosylase (UDG) catalyzes the release of uracil from uracil-containing single-stranded or double-stranded DNA, but not from RNA or oligonucleotides (6 or fewer bases).
UDG is active over a broad pH range with an optimum at pH 8.0, does not require a divalent cation, and is inhibited by high ionic strength (>200 mM).
Meridian UDG is purified to SDS-PAGE purity and is free of endonucleases, exonucleases, nickases and RNases.
Source: Recombinant E. coli strain carrying the over expressed gene of Uracil DNA Glycosylase from E. coli.
Applications
- Control of carry-over contamination in PCR
- As a probe for protein-DNA interaction studies
- Cloning of PCR products
Bioline美国BIOLINE 美国BIOLINE公司：分子生物学试剂，生产以重组的脂质体转染试剂及PCR和RT-PCR产品闻名于世。成立于1992年，总部位于分子生物学领域的出色质量和可靠性试剂，包括dNTP，聚合酶和定量梯
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